Human/Mouse FLIP Antibody

R&D Systems, part of Bio-Techne | Catalog # AF821

R&D Systems, part of Bio-Techne
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AF821
AF821-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Porcine

Applications

Validated:

Western Blot

Cited:

Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all FLIP Antibodies »

Product Specifications

Immunogen

KLH-coupled mouse FLIP synthetic peptide
LKQGRRRPLVDLHVELMDKC

Specificity

Detects human and mouse FLIP in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse FLIP Antibody

Detection of Human/Mouse FLIP antibody by Western Blot.

Detection of Human/Mouse FLIP by Western Blot.

Western blot shows lysates of WS-1 human fetal skin fibroblast cell line and L-929 mouse fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse FLIP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF821) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for FLIP at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse FLIP Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: WS-1 human fetal skin fibroblast cell line and L-929 mouse fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FLIP

FLIP (Flice-like Inhibitory Protein), also known as I-FLICE, CASPER, and FLAME-1, is an apoptosis inhibitory protein with architecture similar to that of Caspases-8 and -10. Both of the FLIP isoforms, Long (L) and Short (S), contain two death effector domains (DED). FLIP(L) has a C-terminal Caspase-like protease domain which lacks both a catalytic active site and residues that form a substrate-binding pocket. FLIP(S) and FLIP(L) interact with the adaptor molecule FADD, and potently inhibit apoptosis initiated by Fas Ligand/TNF signaling pathways.

Long Name

FLICE Inhibitory Proteins

Alternate Names

CASPER, CFLAR, FLAME-1, I-FLICE

Entrez Gene IDs

8837 (Human); 12633 (Mouse)

Gene Symbol

CFLAR

Additional FLIP Products

Product Documents for Human/Mouse FLIP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse FLIP Antibody

For research use only

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