Human/Mouse GATA-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB17791
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 234737
Product Specifications
Immunogen
E. coli-derived recombinant human GATA-1
Met1-Ser413
Accession # P15976
Met1-Ser413
Accession # P15976
Specificity
Detects endogenous human and mouse GATA-1 in Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human/Mouse GATA-1 Antibody
Detection of Human/Mouse GATA-1 by Western Blot.
Western blot shows lysates of TS1 mouse helper T cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse GATA-1 Monoclonal Antibody (Catalog # MAB17791) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for GATA-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.Applications for Human/Mouse GATA-1 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: TS1 mouse helper T cell line and K562 human chronic myelogenous leukemia cell line
Sample: TS1 mouse helper T cell line and K562 human chronic myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GATA-1
GATA-1 is the founding member of the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue‑specific programs of gene expression and morphogenesis. GATA-1 is expressed in blood forming cells. It interacts with several additional proteins to activate or repress gene expression and is essential for erythropoiesis.
Additional GATA-1 Products
Product Documents for Human/Mouse GATA-1 Antibody
Product Specific Notices for Human/Mouse GATA-1 Antibody
For research use only
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