Human/Mouse Haptoglobin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8465
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Human Haptoglobin from human serum
Specificity
Detects human and mouse Haptoglobin in Western blots. In sandwich immunoassays, human Haptoglobin is detected.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse Haptoglobin Antibody
Detection of Human and Mouse Haptoglobin by Western Blot.
Western blot shows human plasma. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8465) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Haptoglobin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human and Mouse Haptoglobin by Western Blot.
Western blot shows lysates of human Haptoglobin purified from human serum and mouse haptoglobin purified from mouse serum. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8465) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Haptoglobin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Haptoglobin by Simple WesternTM.
Simple Western lane view shows human serum, loaded at 1:25000 dilution. A specific band was detected for Haptoglobin at approximately 60 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8465) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse Haptoglobin Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Haptoglobin Monoclonal Antibody (Catalog # MAB8465).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Haptoglobin DuoSet ELISA Kit (Catalog # DY8465-05) for convenient development of a sandwich ELISA or the Human Haptoglobin Quantikine ELISA Kit (Catalog # DHAPG0) for a complete optimized ELISA.
Simple Western
1 µg/mL
Sample: Human Serum
Sample: Human Serum
Western Blot
0.2 µg/mL
Sample: Human Plasma, Human Haptoglobin purified from human serum, and Mouse haptoglobin purified from mouse serum
Sample: Human Plasma, Human Haptoglobin purified from human serum, and Mouse haptoglobin purified from mouse serum
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Haptoglobin
Haptoglobin is a 406 aminoacids (aa) protein that in humans is encoded by the HP gene. This gene encodes a preproprotein that is processed into alpha (aa 19-160) and beta (aa 162-406) chains that combine as a tetramer to produce haptoglobin. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage.
Alternate Names
HP, HP-1, hp2-alpha, HPA1S
Gene Symbol
HP
Additional Haptoglobin Products
Product Documents for Human/Mouse Haptoglobin Antibody
Product Specific Notices for Human/Mouse Haptoglobin Antibody
For research use only
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