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Human/Mouse HMGA2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3184

R&D Systems, part of Bio-Techne
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AF3184
AF3184-SP

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Canine

Applications

Validated:

Immunocytochemistry, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant human HMGA2
Ser2-Asp109
Accession # P52926

Specificity

Detects human and mouse HMGA2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human HMGB1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse HMGA2 Antibody

Detection of Human and Mouse HMGA2 antibody by Western Blot.

Detection of Human and Mouse HMGA2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MDA-MD-231 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and P19 mouse embryonal carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse HMGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3184) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HMGA2 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

HMGA2 in IMR-90 Human Cell Line.

HMGA2 was detected in immersion fixed IMR-90 human lung fibroblast cell line using Goat Anti-Human/Mouse HMGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3184) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Mouse HMGA2 antibody by Simple WesternTM.

Detection of Mouse HMGA2 by Simple WesternTM.

Simple Western lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HMGA2 at approximately 30 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse HMGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3184) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse HMGA2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed IMR-90 human lung fibroblast cell line

Simple Western

10 µg/mL
Sample: NIH‑3T3 mouse embryonic fibroblast cell line

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, MDA‑MD‑231 human breast cancer cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and P19 mouse embryonal carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HMGA2

HMGA2, previously known as HMGI-C, belongs to the group of high mobility chromosomal proteins that have the AT-hook DNA-binding motif. It is highly and ubiquitously expressed during embryogenesis. Chromosomal rearrangements of the HMGA2 gene and overexpression are frequently associated with benign and malignant tumors, respectively. Human and mouse HMGA2 share 95% amino acid sequence identity.

Long Name

High Mobility Group AT-hook 2

Alternate Names

BABL, HMGIC, LIPO

Entrez Gene IDs

8091 (Human)

Gene Symbol

HMGA2

UniProt

Additional HMGA2 Products

Product Documents for Human/Mouse HMGA2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse HMGA2 Antibody

For research use only

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