Human/Mouse HO-1/HMOX1/HSP32 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3776
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Xenograft
Applications
Validated:
Knockout Validated, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 412811
Product Specifications
Immunogen
E. coli-derived recombinant human HO-1/HMOX1
Met1-Thr261
Accession # P09601
Met1-Thr261
Accession # P09601
Specificity
Detects endogenous human and mouse HO-1 in Western blots. In Western blots, this antibody does not cross‑react with rhHO-2.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human/Mouse HO-1/HMOX1/HSP32 Antibody
Detection of Human/Mouse HO-1/HMOX1/HSP32 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse HO-1/HMOX1/HSP32 Monoclonal Antibody (Catalog # MAB3776) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Western Blot Shows Human HO‑1/HMOX1/HSP32 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HO-1/HMOX1/HSP32 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse HO-1/HMOX1/HSP32 Monoclonal Antibody (Catalog # MAB3776) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse HO-1/HMOX1/HSP32 Antibody
Application
Recommended Usage
Knockout Validated
HO‑1/HMOX1/HSP32
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
HO‑1/HMOX1/HSP32 knockout HeLa cell line.
Western Blot
1 µg/mL
Sample: A549 human lung carcinoma cell line and A20 mouse B cell lymphoma cell line
Sample: A549 human lung carcinoma cell line and A20 mouse B cell lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HO-1/HMOX1/HSP32
Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme Oxygenase occurs as 2 isozymes, an inducible Heme Oxygenase-1 (HO-1/HMOX1) and a constitutive Heme Oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Human HO-1 shares 82% amino acid sequence identity with mouse HO-1.
Long Name
Heme Oxygenase 1
Alternate Names
HMOX1, HO1, HSP32
Gene Symbol
HMOX1
UniProt
Additional HO-1/HMOX1/HSP32 Products
Product Documents for Human/Mouse HO-1/HMOX1/HSP32 Antibody
Product Specific Notices for Human/Mouse HO-1/HMOX1/HSP32 Antibody
For research use only
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