Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human/Mouse IL-12/IL-35 p35 PE-conjugated Antibody

Detection of IL‑12/IL‑35 p35 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) either untreated (orange open histogram) or treated with LPS (orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 PE-conjugated Monoclonal Antibody (Catalog # IC2191P) or isotype control antibody (Catalog # IC002P, blue open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of IL‑12/IL‑35 p35 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes either untreated (orange open histogram) or treated with LPS (orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 PE-conjugated Monoclonal Antibody (Catalog # IC2191P) or isotype control antibody (Catalog # IC002P, blue open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse IL-12/IL-35 p35 PE-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs) and mouse splenocytes treated with LPS were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-12/IL-35 p35

Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3⁺ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3⁺ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).

References

Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
Collison, L.W. et al. (2007) Nature 450:566.
Bardel, E. et al. (2008) J. Immunol. 181:6898.
Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.

Long Name

Interleukin 12 p35

Alternate Names

IL-12A, IL35p35

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat)

Gene Symbol

IL12A

UniProt

P29459

Additional IL-12/IL-35 p35 Products

Product Documents for Human/Mouse IL-12/IL-35 p35 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse IL-12/IL-35 p35 PE-conjugated Antibody

For research use only

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Flow Cytometry

Human/Mouse IL-12/IL-35 p35 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC2191P

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications