Human/Mouse IRF6 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6114
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 503404
Product Specifications
Immunogen
E. coli-derived recombinant human IRF6
Ala176-Gln366
Accession # O14896
Ala176-Gln366
Accession # O14896
Specificity
Detects human and mouse IRF6 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse IRF6 Antibody
Detection of Human and Mouse IRF6 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and C2C12 mouse myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse IRF6 Monoclonal Antibody (Catalog # MAB6114) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IRF6 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse IRF6 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and C2C12 mouse myoblast cell line
Sample: K562 human chronic myelogenous leukemia cell line and C2C12 mouse myoblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IRF6
Interferon regulatory factor 6 (IRF6), is a transcription factor that controls the proliferation and differentiation of epidermal and epithelial cells. IRF6 interacts with maspin in normal mammary epithelial cells but is down‑regulated during tumor progression. IRF6 polymorphisms cause Van der Woude and popliteal pterygium syndromes of skin, limb, and craniofacial development that are characterized by cleft lip, cleft palate, or lip pits. IRF6 contains an N-terminal DNA binding domain that is homologous to that in other IRF proteins. Within aa 176‑366 of the C‑terminal domain, human IRF6 shares 98% aa sequence identity with mouse and rat IRF6.
Long Name
Interferon Regulatory Factor 8
Alternate Names
LPS, OFC6, PIT, PPS, VWS
Gene Symbol
IRF6
UniProt
Additional IRF6 Products
Product Documents for Human/Mouse IRF6 Antibody
Product Specific Notices for Human/Mouse IRF6 Antibody
For research use only
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