HEK293 human embryonic kidney cell line transfected with (A) Layilin or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human/Mouse Layilin Monoclonal Antibody (Catalog # MAB2637) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse Layilin Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human Layilin and eGFP

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Layilin (Catalog # 2536‑LA), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Layilin (Catalog # 2536-LA)
Recombinant Mouse Layilin (Catalog # 2646-LA)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Layilin

Layilin is a type I transmembrane protein that acts as a receptor for hyaluronan. It has an extracellular C-type lectin domain and a 123 aa cytoplasmic domain that interacts with the cytoskeleton. The amino acid sequence of recombinant human Layilin is 85%, 82%, 80%, 79% and 76% identical to that of hamster, canine, bovine, mouse and rat.

Alternate Names

LAYN, LYAN

Entrez Gene IDs

143903 (Human); 244864 (Mouse)

Gene Symbol

LAYN

UniProt

NP_849156

Additional Layilin Products

Product Documents for Human/Mouse Layilin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human/Mouse Layilin Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human/Mouse Layilin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2637

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Layilin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse Layilin Antibody

Detection of Layilin in HEK293 Human Cell Line Transfected with Human Layilin and eGFP by Flow Cytometry.