Human/Mouse Lumican Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB28461
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 669401
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Lumican
Gln19-Asn338
Accession # NP_002336
Gln19-Asn338
Accession # NP_002336
Specificity
Detects human and mouse Lumican in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse Lumican Antibody
Detection of Human Lumican by Western Blot.
Western blot shows lysates of human cartilage tissue. PVDF Membrane was probed with 2 µg/mL of Human Lumican Monoclonal Antibody (Catalog # MAB28461) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Differentially glycosylated Lumican protein species were detected between 65 and 85 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse Lumican Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human cartilage tissue
Sample: Human cartilage tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Lumican
Lumican is a secreted matrix protein that belongs to the small leucine‑rich proteoglycan (SLRP) class II family. Mature Lumican has a negatively charged N‑terminal domain that contains tyrosine sulfates, followed by twelve leucine‑rich repeats, which are flanked by cysteine loops. Lumican is expressed widely in mammalian connective tissues, while corneal Lumican exists as a keratan sulfate proteoglycan in adult cartilage, Lumican exists primarily as a glycoprotein and lacks keratan sulfate. The amino acid sequence of human Lumican shares 88% amino acid sequence identity with that of mature mouse Lumican.
Alternate Names
LDC, LUM, SLRR2D
Gene Symbol
LUM
UniProt
Additional Lumican Products
Product Documents for Human/Mouse Lumican Antibody
Product Specific Notices for Human/Mouse Lumican Antibody
For research use only
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