Human/Mouse MafK Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3809
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 381923
Product Specifications
Immunogen
E. coli-derived recombinant human MafK
Thr2-Ser156
Accession # O60675
Thr2-Ser156
Accession # O60675
Specificity
Detects human and mouse MafK in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MafB or rhMafF is observed. In Western blots, prolonged film exposures may slightly detect rhMafG.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse MafK Antibody
Detection of Human/Mouse MafK by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human/Mouse MafK Monoclonal Antibody (Catalog # MAB3809) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MafB, MafF, MafG, and MafK (0.5 ng/lane) were included. A specific band for MafK was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human/Mouse MafK Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: A549 human lung carcinoma cell line
Sample: A549 human lung carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MafK
Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including c‑Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK, also known as p18. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members. Heterodimers of MafK and p45 NF-E2, another bZIP factor, promote globin gene expression and erythroid differentiation in erythroleukemia cells.
Long Name
v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog K
Alternate Names
NFE2U
Gene Symbol
MAFK
UniProt
Additional MafK Products
Product Documents for Human/Mouse MafK Antibody
Product Specific Notices for Human/Mouse MafK Antibody
For research use only
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