Human/Mouse MBD4 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5935
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human MBD4
Met382-Ser580
Accession # O95243
Met382-Ser580
Accession # O95243
Specificity
Detects human and mouse MBD4 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse MBD4 Antibody
Detection of Human and Mouse MBD4 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse MBD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5935) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MBD4 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse MBD4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line
Sample: HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MBD4
Long Name
Methyl-CpG Binding Domain Protein 4
Alternate Names
EC 3.2.2.-, G/T mismatch glycosylase, G/U mismatch glycosylase, MED1G/5-fluorouracil mismatch glycosylase with biphasic kinetics, methyl-CpG binding domain protein 4, methyl-CpG-binding domain protein 43,N(4)-ethenocytosine glycosylase, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase, putative methyl-CpG binding protein
Gene Symbol
MBD4
UniProt
Additional MBD4 Products
Product Documents for Human/Mouse MBD4 Antibody
Product Specific Notices for Human/Mouse MBD4 Antibody
For research use only
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