Human/Mouse Neprilysin/CD10 Antibody

Neprilysin/CD10 in Human Kidney.

Neprilysin/CD10 was detected in immersion fixed paraffin-embedded sections of human kidney using Rat Anti-Human/Mouse Neprilysin/CD10 Monoclonal Antibody (Catalog # MAB1126) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules and glomeruli. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human and Mouse Neprilysin/CD10 by Western Blot.

Western blot shows lysates of LNCaP human prostate cancer cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse Neprilysin/CD10 Monoclonal Antibody (Catalog # MAB1126) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, Recombinant Mouse Neprilysin (Catalog # 1126-ZN) (5 and 50 ng/lane) and Recombinant Human Neprilysin (CHO-expressed) (Catalog # 1182-ZNC) (5 and 50 ng/lane) were included. Specific bands were detected for Neprilysin/CD10 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.