Human/Mouse NPM1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5205
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NPM1
Met9-Leu158
Accession # P06748
Met9-Leu158
Accession # P06748
Specificity
Detects human and mouse NPM1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse NPM1 Antibody
Detection of Human and Mouse NPM1 by Western Blot.
Western blot shows lysates of Nalm-6 human Pre-B acute lymphocytic leukemia cell line, Jurkat human acute T cell leukemia cell line and mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse NPM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5205) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NPM1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human/Mouse NPM1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Nalm-6 human Pre-B acute lymphocytic leukemia cell line, Jurkat human acute T cell leukemia cell line and mouse spleen tissue
Sample: Nalm-6 human Pre-B acute lymphocytic leukemia cell line, Jurkat human acute T cell leukemia cell line and mouse spleen tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NPM1
NPM1 (Nucleophosmin 1; also nucleolar protein 23) is a 40 kDa member of the nucleoplasmin family of proteins. It is found in the nucleus where it performs multiple functions. NPM1 normally associates with unduplicated centrosomes. After phosphorylation at Thr199, it dissociates from centrosomes, allowing for centrosome duplication. NPM1 also negatively regulates GCN5-dependent histone acetylation during mitosis. Human NPM1 is 294 amino acids (aa) in length. It contains a Met-rich region (aa 1‑9), two acidic regions (aa 120‑132 and 161‑188) and two NLSs (aa 152‑157 and 191‑197). Phosphorylation occurs at Ser4/10/70/254. Splice variants show a deletion of aa 195‑223, a two aa substitution for aa 258‑294 and an 11 aa substitution for aa 288‑294. Over aa 9‑158, human NPM1 is 97% aa identical to mouse NPM1.
Long Name
Nucleophosmin/Nucleolar Phosphoprotein B23
Alternate Names
B23, Nucleophosmin, Numatrin
Gene Symbol
NPM1
UniProt
Additional NPM1 Products
Product Documents for Human/Mouse NPM1 Antibody
Product Specific Notices for Human/Mouse NPM1 Antibody
For research use only
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