BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 APC-conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Detection of Oct-3/4 in D3 Mouse Cell Line by Flow Cytometry.

D3 mouse embryonic stem cell line treated with 5 µM Retinoic Acid for 3 days was stained with Rat Anti-Human/Mouse Oct-3/4 APC-conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Detection of Oct-3/4 in iPSC cells by Flow Cytometry.

iPSC cells were stained with Rat Anti-Human/Mouse Oct-3/4 APC-conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse Oct-3/4 APC-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: BG01V human embryonic stem cells and D3 mouse embryonic stem cell line treated with Retinoic Acid were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) and iPSC cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oct-3/4

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

References

Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
Scholer, H.R. et al. (1989) EMBO J. 8:2543.
Rosner, M.H. et al. (1990) Nature 345:686.
Niwa, H. et al. (2000) Nat. Genet. 24:372.

Long Name

Octamer-binding Protein 3/4

Alternate Names

3-Oct, Oct4, Otf3g, Otf4, Pou5f1

Entrez Gene IDs

5460 (Human); 18999 (Mouse)

Gene Symbol

POU5F1

UniProt

Q01860

Additional Oct-3/4 Products

Product Documents for Human/Mouse Oct-3/4 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human/Mouse Oct-3/4 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human/Mouse Oct-3/4 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC1759A

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Oct-3/4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse Oct-3/4 APC-conjugated Antibody

Detection of Oct-3/4 in BG01V Human Cells by Flow Cytometry.