NTERA-2 cells were stained with Rat Anti-Human/Mouse Oct-3/4 APC-conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Detection of Oct-3/4 in BG01V Human Cells by Flow Cytometry.

BG01V human embryonic stem cells were stained with Rat Anti-Human/Mouse Oct-3/4 APC-conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (IC013A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Detection of Oct-3/4 in iPSC cells by Flow Cytometry.

iPSC cells were stained with Rat Anti-Human/Mouse Oct-3/4 APC-conjugated Monoclonal Antibody (Catalog # IC1759A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Applications for Human/Mouse Oct-3/4 APC-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: NTERA-2 human testicular embryonic carcinoma cell line or BG01V human embryonic stem cells and D3 mouse embryonic stem cell line treated with Retinoic Acid were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) and iPSC cells fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oct-3/4

Oct-3/4, also termed Oct-3 or Oct-4, is a POU transcription factor that is expressed in totipotent embryonic stem and germ cells.(2, 3) Oct-3/4 is required to sustain stem cell self-renewal and pluripotency.(4) It is considered a master regulator of pluripotency that controls lineage commitment and is the most widely recognized marker of totipotent embryonic stem cells.

References

Takeda, J. et al. (1992) Nucleic Acids Res. 20:4613.
Scholer, H.R. et al. (1989) EMBO J. 8:2543.
Rosner, M.H. et al. (1990) Nature 345:686.
Niwa, H. et al. (2000) Nat. Genet. 24:372.

Long Name

Octamer-binding Protein 3/4

Alternate Names

3-Oct, Oct4, Otf3g, Otf4, Pou5f1

Entrez Gene IDs

5460 (Human); 18999 (Mouse)

Gene Symbol

POU5F1

UniProt

Q01860

Additional Oct-3/4 Products

Product Documents for Human/Mouse Oct-3/4 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human/Mouse Oct-3/4 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human/Mouse Oct-3/4 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC1759A

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Oct-3/4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human/Mouse Oct-3/4 APC-conjugated Antibody

Detection of Oct-3/4 in NTERA-2 cells by Flow Cytometry