Human/Mouse PBEF/Visfatin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4044
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 362616
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PBEF/Visfatin
Met1-His491
Accession # Q99KQ4
Met1-His491
Accession # Q99KQ4
Specificity
Detects human and mouse PBEF/Visfatin in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human PBEF is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human/Mouse PBEF/Visfatin Antibody
Detection of PBEF/Visfatin in 3T3‑L1 Mouse Cell Line by Flow Cytometry.
3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Rat Anti-Human/Mouse PBEF/ Visfatin Monoclonal Antibody (Catalog # MAB4044, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human/Mouse PBEF/Visfatin Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: 3T3-L1 mouse embryonic fibroblast adipose-like cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: 3T3-L1 mouse embryonic fibroblast adipose-like cell line fixed with paraformaldehyde and permeabilized with saponin
Western Blot
1 µg/mL
Sample: Recombinant Human and mouse PBEF
Sample: Recombinant Human and mouse PBEF
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PBEF/Visfatin
Long Name
Pre-B-Cell Colony-Enhancing Factor 1
Alternate Names
NAmPRTase, NAMPT, NMPRTase, PBEF1, Visfatin
Gene Symbol
NAMPT
UniProt
Additional PBEF/Visfatin Products
Product Documents for Human/Mouse PBEF/Visfatin Antibody
Product Specific Notices for Human/Mouse PBEF/Visfatin Antibody
For research use only
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