Human/Mouse Phospho-ERK1 (ERK1 T202/Y204, ERK2 T185/Y187) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB18251

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB18251-100
MAB18251-SP
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1088B

View all ERK1 Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human ERK1 T202/Y204  site
Accession # P27361

Specificity

Detects human and mouse ERK1 and ERK2 dually phosphorylated at T202/Y204 or T185/Y187, respectively.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse Phospho-ERK1 (ERK1 T202/Y204, ERK2 T185/Y187) Antibody

Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) antibody by Western Blot.

Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Monoclonal Antibody (Catalog # MAB18251) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204) at approximately 44 kDa and Phospho-ERK2 (T185/Y187) at approximately 42 (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) antibody by Western Blot.

Detection of Mouse Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Western Blot.

Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 5 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Monoclonal Antibody (Catalog # MAB18251) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204) at approximately 44 kDa and Phospho-ERK2 (T185/Y187) at approximately 42 (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) antibody by Simple WesternTM.

Detection of Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with PMA and Ionomycin, loaded at 0.2 mg/mL. Specific bands were detected for Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) at approximately 46-49 kDa (as indicated) using 1 μg/mL of Rabbit Anti-Human/Mouse Phospho-ERK1 (ERK1 T202/Y204, ERK2 T185/Y187) Monoclonal Antibody (Catalog # MAB18251) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse Phospho-ERK1 (ERK1 T202/Y204, ERK2 T185/Y187) Antibody

Application
Recommended Usage

Simple Western

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with PMA and Ionomycin

Western Blot

0.1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with PMA and NIH‑3T3 mouse embryonic fibroblast cell line treated with Recombinant Human PDGF‑BB (Catalog # 220-BB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB18251 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK1

ERK1 and ERK2 (also known as MAPK3 and MAPK1) are 44- and 42-kDa Ser/Thr kinases, respectively. They are part of the Ras-Raf-ERK signal transduction cascade often found downstream of growth factor receptor activation. ERK1 and ERK2 were initially isolated and cloned as kinases activated in response to insulin and NGF. They are expressed in most, if not all, mammalian tissues. Dual threonine and tyrosine phosphorylation activate both ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2. Within the range used as an immunogen, human, mouse, and rat ERK1 share 100% amino acid sequence identity.

Long Name

Extracellular Signal-regulated Kinase 1

Alternate Names

MAPK3, P44ERK1, p44mapk, PRKM3

Entrez Gene IDs

5595 (Human); 26417 (Mouse); 50689 (Rat)

Gene Symbol

MAPK3

UniProt

P27361

Additional ERK1 Products

Product Documents for Human/Mouse Phospho-ERK1 (ERK1 T202/Y204, ERK2 T185/Y187) Antibody

Product Datasheets

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Product Specific Notices for Human/Mouse Phospho-ERK1 (ERK1 T202/Y204, ERK2 T185/Y187) Antibody

For research use only

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