Human/Mouse Phospho-RSK1 (S221)/RSK2 (S227) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB892
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1019B
Product Summary for Human/Mouse Phospho-RSK1 (S221)/RSK2 (S227) Antibody
Immunogen
Phosphopeptide containing the human RSK1 S221 site
Specificity
Detects human and mouse RSK1 when phosphorylated at S221, and human and mouse RSK2 when phosphorylated at S227.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse Phospho-RSK1 (S221)/RSK2 (S227) Antibody
Detection of Human Phospho-RSK1 (S221)/RSK2 (S227) by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Monoclonal Antibody (Catalog # MAB892) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phopho-RSK1 (S221)/RSK2 (S227) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Phospho-RSK1 (S221)/RSK2 (S227) by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes, loaded at 0.1 mg/mL. A specific band was detected for Phospho-RSK1 (S221)/RSK2 (S227) at approximately 91 kDa (as indicated) using 5 μg/mL of Rabbit Anti-Human/Mouse Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227) Monoclonal Antibody (Catalog # MAB892) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse Phospho-RSK1 (S221)/RSK2 (S227) Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with PMA
Sample: HeLa human cervical epithelial carcinoma cell line treated with PMA
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with PMA
Sample: HeLa human cervical epithelial carcinoma cell line treated with PMA
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB892 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RSK1/RSK2
Long Name
Ribosomal Protein S6 Kinase II
Additional RSK1/RSK2 Products
Product Documents for Human/Mouse Phospho-RSK1 (S221)/RSK2 (S227) Antibody
Product Specific Notices for Human/Mouse Phospho-RSK1 (S221)/RSK2 (S227) Antibody
For research use only
Loading...
Loading...
Loading...
Loading...