Human/Mouse PKC iota/ lambda Antibody

Members of the Protein Kinase C (PKC) family are serine/threonine protein kinases that play a key regulatory role in a number of cellular functions including cell growth and differentiation, hormone secretion, and gene expression. Multiple genes and alternative splicing result in three subfamilies, which differ in their cofactor requirements: conventional PKC isoforms ( alpha, beta_Ι, beta_II, and gamma) which require calcium and phosphatidylserine (PS), diacylglycerol (DAG) or phorbol esters for activation; novel isoforms ( delta, epsilon, eta, and theta), which are calcium-independent but are still regulated by PS, DAG, or phorbol esters; and atypical isoforms ( iota / lambda, and zeta), which are calcium-independent and do not require PS, DAG, or phorbol esters for activation. PKC iota is highly expressed in brain and lung but is also expressed at lower levels in many tissues including pancreatic islets. It is the catalytic component of the Par3-Par6-atypical PKC complex, a key regulator of the formation and maintenance of epithelial cell polarity, adherens junctions, and tight junctions. PKC iota has also been shown to be critical for Ras-mediated transformation, invasion, and anchorage‑independent growth of intestinal epithelial cells. The rodent homolog is termed PKC lambda and is 95% homologous to PKC iota.