Human/Mouse PLC-gamma 2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3716
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 346404
Product Specifications
Immunogen
E. coli-derived recombinant human PLC-gamma 2
Pro400-Tyr600
Accession # P16885
Pro400-Tyr600
Accession # P16885
Specificity
Detects human and mouse PLC-gamma 2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse PLC-gamma 2 Antibody
Detection of Human/Mouse PLC-gamma 2 by Western Blot.
Western blot shows lysates of A20 mouse B cell lymphoma cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse PLC- gamma2 Monoclonal Antibody (Catalog # MAB3716) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PLC- gamma2 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PLC-gamma 2 in Human PBMCs.
PLC- gamma2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human/Mouse PLC- gamma2 Monoclonal Antibody (Catalog # MAB3716) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Mouse PLC-gamma 2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells
Sample: Immersion fixed human peripheral blood mononuclear cells
Western Blot
1 µg/mL
Sample: A20 mouse B cell lymphoma cell line and U937 human histiocytic lymphoma cell line
Sample: A20 mouse B cell lymphoma cell line and U937 human histiocytic lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB3716 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLC-gamma 2
The Phospholipase C family consists of 13 isozymes within six subfamilies, PLC-delta, -beta, -gamma, -epsilon, -zeta, and -eta. PLC-gamma 2 (Phospholipase C gamma-2) contains 2 SH2 and 1 SH3 domains and is primarily limited to cells of hematopoietic lineage. PLC- gamma2 is activated by receptor tyrosine kinases in response to growth factors, neurotransmitters, and hormones. Activated PLC- gamma2 catalyzes the hydrolysis of phosphatidylinositol 4, 5-biphosphate to produce the second messengers inositol 1, 4, 5-triphosphate (IP3) and diacylglycerol (DAG). IP3 mobilizes the release of calcium while DAG activates protein kinase C. PLC- gamma2 is involved in collagen induced signaling in platelets and antigen-dependent signaling in B-lymphocytes.
Long Name
Phospholipase C gamma 2
Alternate Names
PLCG2, PLCgamma 2
Gene Symbol
PLCG2
UniProt
Additional PLC-gamma 2 Products
Product Documents for Human/Mouse PLC-gamma 2 Antibody
Product Specific Notices for Human/Mouse PLC-gamma 2 Antibody
For research use only
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