Human/Mouse Plexin A2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5486
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 583603
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin A2
Met35-Pro1237
Accession # P70207
Met35-Pro1237
Accession # P70207
Specificity
Detects mouse Plexin A2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Plexin A4, B1, B2, D1, recombinant mouse Plexin A1, A3, B3, or C1 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human/Mouse Plexin A2 Antibody
Detection of Mouse Plexin A2 by Western Blot.
Western blot shows lysates of mouse brain tissue. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse Plexin A2 Monoclonal Antibody (Catalog # MAB5486) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Plexin A2 at approximately 200 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Plexin A2 in bEnd.3 Mouse Cell Line.
Plexin A2 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse Plexin A2 Monoclonal Antibody (Catalog # MAB5486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Plexin A2 in HUVEC Human Cells.
Plexin A2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Mouse Plexin A2 Monoclonal Antibody (Catalog # MAB5486) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse Plexin A2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed bEnd.3 mouse endothelioma cell line and HUVEC human umbilical vein endothelial cells
Sample: Immersion fixed bEnd.3 mouse endothelioma cell line and HUVEC human umbilical vein endothelial cells
Western Blot
2 µg/mL
Sample: Mouse brain tissue under non-reducing conditions only
Sample: Mouse brain tissue under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plexin A2
Alternate Names
OCT, PLXN2, PLXNA2
Gene Symbol
PLXNA2
UniProt
Additional Plexin A2 Products
Product Documents for Human/Mouse Plexin A2 Antibody
Product Specific Notices for Human/Mouse Plexin A2 Antibody
For research use only
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