Human/Mouse PSMB7 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7590
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 787807
Product Specifications
Immunogen
E. coli-derived recombinant human PSMB7
Thr44-Ser277
Accession # Q99436
Thr44-Ser277
Accession # Q99436
Specificity
Detects human PSMB7 in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant human PSMB10 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse PSMB7 Antibody
Detection of Human and Mouse PSMB7 by Western Blot.
Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line, betaTC-6 mouse beta cell insulinoma cell line, AR42J human acinar pancreatic cancer cell line, HepG2 human hepatocellular carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, MCF-7 human breast cancer cell line, and NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human PSMB7 Monoclonal Antibody (Catalog # MAB7590) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PSMB7 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse PSMB7 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: 786‑O human renal cell adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and HepG2 human hepatocellular carcinoma cell line
Sample: 786‑O human renal cell adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and HepG2 human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PSMB7
Long Name
Proteasome (Prosome, Macropain) Subunit, beta Type, 7
Alternate Names
Macropain Chain Z, Proteasome Subunit Z
Gene Symbol
PSMB7
UniProt
Additional PSMB7 Products
Product Documents for Human/Mouse PSMB7 Antibody
Product Specific Notices for Human/Mouse PSMB7 Antibody
For research use only
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