Human/Mouse/Rat Akt1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1775
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 232790
Product Specifications
Immunogen
E. coli-derived recombinant human Akt1
Ser2-Ala480
Accession # P31749
Ser2-Ala480
Accession # P31749
Specificity
Detects human, mouse, and rat Akt1 in Western blots. In Western blots, no cross-reactivity with recombinant human Akt2 or Akt3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat Akt1 Antibody
Detection of Human Akt1 by Western Blot.
Western blot shows recombinant human Akt1, Akt2, and Akt3 (5 ng/lane). PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat Akt1 Monoclonal Antibody (Catalog # MAB1775) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Akt1 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of Human, Mouse, and Rat Akt1 by Western Blot.
Western blot shows lysates of MBA-MB-123 human breast cancer cell line, C2C12 mouse myoblast cell line, TS1 mouse helper T cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat Akt1 Monoclonal Antibody (Catalog # MAB1775) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Akt1 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western Blot Shows Human Akt1 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Atk1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat Akt1 Monoclonal Antibody (Catalog # MAB1775) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Akt1 at approximately 72 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Akt1 Antibody
Application
Recommended Usage
Knockout Validated
Akt1
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
Akt1 knockout HeLa cell line.
Western Blot
0.2 µg/mL
Sample: MBA-MB-123 human breast cancer cell line, C2C12 mouse myoblast cell line, TS1 mouse helper T cell line, and C6 rat glioma cell line
Sample: MBA-MB-123 human breast cancer cell line, C2C12 mouse myoblast cell line, TS1 mouse helper T cell line, and C6 rat glioma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Akt1
Akt, also known as protein kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha), Akt2 (PKB beta), and Akt3 (PKB gamma). Akt1 is the most ubiquitously expressed family member. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.
Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog 1
Alternate Names
PKB alpha, PRKBA, RAC-alpha
Gene Symbol
AKT1
UniProt
Additional Akt1 Products
Product Documents for Human/Mouse/Rat Akt1 Antibody
Product Specific Notices for Human/Mouse/Rat Akt1 Antibody
For research use only
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