Human/Mouse/Rat Akt1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF17751
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Akt1
Met1-Glu149
Accession # P31749
Met1-Glu149
Accession # P31749
Specificity
Detects human, mouse, and rat Akt1 in Western blots. The antibody is specific for Akt1 and does not detect recombinant Akt2 or Akt3.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Akt1 Antibody
Detection of Human/Mouse/Rat Akt1 by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line and HL-60 human promyelocytic leukemia cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat Akt1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF17751) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, Recombinant Human Active Akt1 (Catalog # 1775-KS), recombinant human Akt2, and recombinant human Akt3 (2 ng/lane) were included. A specific band for Akt1 was detected at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Akt1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: C2C12 mouse myoblast cell line and HL-60 human promyelocytic leukemia cell line, and Raji human Burkitt's lymphoma cell line
Sample: C2C12 mouse myoblast cell line and HL-60 human promyelocytic leukemia cell line, and Raji human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Akt1
The serine/threonine kinase Akt, also known as protein kinase B (PKB), is a central regulator of such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. In mammals, three highly homologous members define the Akt family: Akt1 (PKB alpha), Akt2 (PKB beta), and Akt3 (PKB gamma). Akt1 is the most ubiquitously expressed family member. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.
Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog 1
Alternate Names
PKB alpha, PRKBA, RAC-alpha
Gene Symbol
AKT1
UniProt
Additional Akt1 Products
Product Documents for Human/Mouse/Rat Akt1 Antibody
Product Specific Notices for Human/Mouse/Rat Akt1 Antibody
For research use only
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