Human/Mouse/Rat APE Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1044
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 200913
Product Specifications
Immunogen
E. coli-derived recombinant human APE
Pro2-Leu318
Accession # P27695
Pro2-Leu318
Accession # P27695
Specificity
Detects human, mouse and rat APE in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat APE Antibody
Detection of Human/Mouse/Rat APE by Western Blot.
Western blot shows lysates of NRK rat normal kidney cell line, Raji human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat APE Monoclonal Antibody (Catalog # MAB1044) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for APE at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat APE Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: NRK rat normal kidney cell, Raji human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line
Sample: NRK rat normal kidney cell, Raji human Burkitt's lymphoma cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: APE
Human APE (also known as Ref-1) is the apurinic/apyrimidinic (AP) endonuclease required for efficient DNA base excision repair (BER). Following the removal of a damaged base by a DNA glycosylase, APE cleaves the AP site to allow resynthesis and ligation to complete repair. In addition, APE/Ref-1 acts as a factor that regulates the redox state of multiple transcription factors, including c-Jun, c-Fos, NF‑ kappaB, and p53.
Long Name
Apurinic/Apyrimidinic Endonuclease
Alternate Names
APEX1
Gene Symbol
APEX1
UniProt
Additional APE Products
Product Documents for Human/Mouse/Rat APE Antibody
Product Specific Notices for Human/Mouse/Rat APE Antibody
For research use only
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