Human/Mouse/Rat ARC Antibody

Name: Human/Mouse/Rat ARC Antibody
Brand: R&D Systems
SKU: AF636

R&D Systems, part of Bio-Techne | Catalog # AF636

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all ARC Antibodies »

Product Specifications

Immunogen

KLH-coupled human ARC synthetic peptide
CEPEAEAEPEPELE PEPDPEP

Specificity

Detects human, mouse, and rat ARC in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat ARC Antibody

Detection of Human/Mouse/Rat ARC by Western Blot.

Western blot shows lysates of rat and mouse skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat ARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF636) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human ARC Western Blot Standard (Catalog # WBC009) (1 ng) was included. A specific band for ARC was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Applications for Human/Mouse/Rat ARC Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hippocampus), heart and skeletal muscle, mouse skeletal muscle, and rat brain

Western Blot

1 µg/mL
Sample: Rat and mouse skeletal muscle tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ARC

ARC [Apoptosis Repressor with CARD (Caspase-recruitment Domain)] is a 29 kDa cytosolic protein containing an N-terminal CARD joined to a C-terminal region rich in proline and glutamic acid. The expression of human ARC is primarily restricted to skeletal muscle and cardiac tissue. ARC inhibits apoptosis induced by Caspase-8.

Long Name

Apoptosis Repressor with Caspase Recruitment Domain

Alternate Names

NOL3

Entrez Gene IDs

8996 (Human); 11838 (Mouse)

Gene Symbol