Human/Mouse/Rat ARK5/NUAK1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6978

R&D Systems, part of Bio-Techne
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MAB6978
MAB6978-SP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 715901

View all ARK5/NUAK1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ARK5/NUAK1
Lys311-Met462
Accession # O60285

Specificity

Detects human ARK5/NUAK1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human SNARK is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat ARK5/NUAK1 Antibody

Detection of Human, Mouse, and Rat ARK5/NUAK1 antibody by Western Blot.

Detection of Human, Mouse, and Rat ARK5/NUAK1 by Western Blot.

Western blot shows lysates of Saos-2 human osteosarcoma cell line, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ARK5/NUAK1 Monoclonal Antibody (Catalog # MAB6978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ARK5/NUAK1 at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat ARK5/NUAK1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Saos‑2 human osteosarcoma cell line, mouse heart tissue, and rat heart tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ARK5/NUAK1

AMPK-related kinase 5 (ARK5), also known as NUAK1, is a 74 kDa intracellular Ser/Thr kinase that promotes cell survival during nutrient deprivation and TNF family ligand-induced apoptosis. ARK5 is activated by Akt, NDR2, and LKB1-mediated phosphorylation at multiple serine and threonine residues. It inhibits the activation of Caspase-6, Caspase-8, and myosin phosphatases but promotes the activation of MMP2 and MMP9. ARK5 overexpression in several cancers contributes to increased tumor progression and aggressiveness. ARK5 contains one catalytic domain (aa 65-306), and a short isoform of the human protein is prematurely tuncated within this domain. Within aa 311-462, human ARK5 shares 88% and 85% aa sequence identity with mouse and rat ARK5, respectively.

Long Name

AMPK-related Protein Kinase 5

Alternate Names

NUAK1

Entrez Gene IDs

9891 (Human); 77976 (Mouse); 299694 (Rat)

Gene Symbol

NUAK1

UniProt

O60285

Additional ARK5/NUAK1 Products

Product Documents for Human/Mouse/Rat ARK5/NUAK1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat ARK5/NUAK1 Antibody

For research use only

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