Human/Mouse/Rat Axin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3287
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Axin-1
Tyr210-Glu410
Accession # O15169
Tyr210-Glu410
Accession # O15169
Specificity
Detects human, mouse and rat Axin-1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Axin-1 Antibody
Detection of Human/Mouse/Rat Axin-1 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Axin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3287) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Axin-1 at approximately 120 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Axin-1 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line
Sample: Raji human Burkitt's lymphoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF3287 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Axin-1
Axin-1 is a negative regulator of the Wnt signaling pathway, which is involved in regulation of embryonic axis formation in vertebrates. Axin-1 binds to beta-catenin and glycogen synthase kinase-3 (GSK-3) to promote beta-catenin phosphorylation and subsequent degradation. Axin-1 also binds to the adenomatous polyposis coli (APC) gene, a known tumor suppressor that also regulates beta-catenin.
Long Name
Axis Inhibitor 1
Alternate Names
Axin1, MGC52315
Gene Symbol
AXIN1
UniProt
Additional Axin-1 Products
Product Documents for Human/Mouse/Rat Axin-1 Antibody
Product Specific Notices for Human/Mouse/Rat Axin-1 Antibody
For research use only
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