Human/Mouse/Rat beta-2 Adrenergic R/ADRB2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10040
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Rat
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2204C
Product Specifications
Immunogen
Synthetic peptide containing human beta‑2 Adrenergic R/ADRB2
Accession # P07550
Accession # P07550
Specificity
Detects human beta‑2 Adrenergic R/ADRB2 in direct ELISAs. Detects human, mouse, and rat beta‑2 Adrenergic R/ADRB2 in Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat beta-2 Adrenergic R/ADRB2 Antibody
Detection of Human, Mouse, and Rat beta‑2 Adrenergic R/ADRB2 by Western Blot.
Western blot shows lysates of human heart tissue, mouse colon tissue, and rat colon tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat beta-2 Adrenergic R/ADRB2 Monoclonal Antibody (Catalog # MAB10040) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for beta-2 Adrenergic R/ADRB2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat beta-2 Adrenergic R/ADRB2 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human heart tissue, Mouse colon tissue, and Rat colon tissue
Sample: Human heart tissue, Mouse colon tissue, and Rat colon tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-2 Adrenergic R/ADRB2
Beta-2 adrenoceptor (ADRB2) is a 413 amino acids adrenergic receptor expressed in smooth muscle and metabolic tissues. Beta-adrenergic receptors belong to the seven-transmembrane domain/GPCR family of receptors and mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Activation of ADRB2 induces a decrease in gastrointestinal motility, bronchodilation, vasodilation in skeletal and cardiac muscle, and glycogenolysis in liver. Agonists of ADRB2 are most widely used for the treatment of asthma. In complex with beta-arrestin-1 and c-src, the beta-2 adrenergic receptor activates MAP kinases ERK1(MAPK3) and ERK2 (MAPK1). Expression of the beta-2 adrenoceptor has been reported in adipose, blood, brain, heart, lung, nose, pancreas, skeletal muscle, skin, and vessel.
Long Name
Beta-2 Adrenergic Receptor
Alternate Names
ADRB2, ADRB2R, B2AR, beta-2 AdrenergicR, beta2 AdrenergicR
Gene Symbol
ADRB2
UniProt
Additional beta-2 Adrenergic R/ADRB2 Products
Product Documents for Human/Mouse/Rat beta-2 Adrenergic R/ADRB2 Antibody
Product Specific Notices for Human/Mouse/Rat beta-2 Adrenergic R/ADRB2 Antibody
For research use only
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