Human/Mouse/Rat ERK2 Antibody

Western Blot Shows Human ERK2 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ERK2 at approximately 42 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse ERK2 by Western Blot

Enhanced Dectin-1 Signaling in Fcer1g−/− DCs. Six-day-cultured BMDCs derived from WT and Fcer1g−/− mice were collected and starved in serum-free RPMI. After 3 h, cells were treated with dZym and lysed at indicated time points. The proteins were separated by SDS-PAGE, transferred to PVDF membranes, and then analyzed by Western blot. The detection of phosphorylated and total proteins of Src, Syk, AKT, PLC gamma, ERK, p38MAPK, and c-Raf (A), and total proteins of I kappaB alpha and GAPDH (B), were shown. Quantification was determined by densitometry using ImageJ software and the number represented the fold of each (phosphoprotein/total protein) value normalized to the (phosphoprotein/total protein) value of untreated WT control (WT 0 min). All data shown were representative from three to five independent experiments. Image collected and cropped by CiteAb from the following publication (https://journal.frontiersin.org/article/10.3389/fimmu.2017.01424/full), licensed under a CC-BY license. Not internally tested by R&D Systems.