Human/Mouse/Rat ERK2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1230
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Knockout Validated, Western Blot
Cited:
Functional Assay
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ERK2
Met1-Ser360
Accession # P28482
Met1-Ser360
Accession # P28482
Specificity
Detects human, mouse, and rat ERK2. Also recognizes ERK1 in some cell types at much lower affinity.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat ERK2 Antibody
Detection of Human/Mouse/Rat ERK2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ERK2 in Human Breast.
ERK2 was detected in immersion fixed paraffin-embedded sections of human breast using Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Western Blot Shows Human ERK2 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and ERK2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1230) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ERK2 at approximately 42 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat ERK2 Antibody
Application
Recommended Usage
Immunohistochemistry
3-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13-15), perfusion fixed frozen sections of rat brain, and immersion fixed paraffin-embedded sections of human breast
Sample: Immersion fixed frozen sections of mouse embryo (E13-15), perfusion fixed frozen sections of rat brain, and immersion fixed paraffin-embedded sections of human breast
Knockout Validated
ERK2
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
ERK2 knockout HeLa cell line.
Western Blot
0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC‑12 rat adrenal pheochromocytoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, PC‑12 rat adrenal pheochromocytoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ERK2
Long Name
Extracellular Signal-regulated Kinase 2
Alternate Names
ERT1, MAPK1, MAPK2, p41mapk, p42mapk, PRKM1, PRKM2
Gene Symbol
MAPK1
UniProt
Additional ERK2 Products
Product Documents for Human/Mouse/Rat ERK2 Antibody
Product Specific Notices for Human/Mouse/Rat ERK2 Antibody
For research use only
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