Human/Mouse/Rat FKBP12.6 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4174
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Canine
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FKBP12.6
Met1-Glu108
Accession # P68106
Met1-Glu108
Accession # P68106
Specificity
Detects human, mouse, and rat FKBP12.6 in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human FKBP12 and less than 1% cross-reactivity with recombinant human FKBP13 and FKBP25 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat FKBP12.6 Antibody
Detection of Human/Mouse/Rat FKBP12.6 by Western Blot.
Western blot shows lysates of human cortex, human hippocampus, and mouse brain tissues. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP12.6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4174) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FKBP12.6 at approximately 13 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Specificity of FKBP12.6 Shown by Western Blot.
Western blot shows recombinant human FKBP 12, recombinant human FKBP 12.6, recombinant human FKBP 13, recombinant human FKBP 25, recombinant human FKBP 38, and recombinant human FKBP 52. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat FKBP12.6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4174) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FKBP12.6 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FKBP12.6 in Human Brain.
FKBP12.6 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Goat Anti-Human/Mouse/Rat FKBP12.6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4174) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse/Rat FKBP12.6 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate nucleus)
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate nucleus)
Simple Western
50 µg/mL
Sample: Human brain (cortex) tissue and mouse brain (cortex) tissue
Sample: Human brain (cortex) tissue and mouse brain (cortex) tissue
Western Blot
1 µg/mL
Sample: DU145 human prostate carcinoma cell line, C2C12 mouse myoblast cell line, Neuro-2A mouse neuroblastoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and Recombinant human FKBP 12.6
Sample: DU145 human prostate carcinoma cell line, C2C12 mouse myoblast cell line, Neuro-2A mouse neuroblastoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and Recombinant human FKBP 12.6
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FKBP12.6
Long Name
12.6 kDa FK506 Binding Protein
Alternate Names
FKBP1B, FKBP1L, FKBP9, OTK4
Entrez Gene IDs
2281 (Human)
Gene Symbol
FKBP1B
UniProt
Additional FKBP12.6 Products
Product Documents for Human/Mouse/Rat FKBP12.6 Antibody
Product Specific Notices for Human/Mouse/Rat FKBP12.6 Antibody
For research use only
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