Human/Mouse/Rat FKBP12.6 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF4174
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FKBP12.6
Met1-Glu108
Accession # P68106
Met1-Glu108
Accession # P68106
Specificity
Detects human, mouse, and rat FKBP12.6 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat FKBP12.6 Biotinylated Antibody
Detection of Human FKBP12.6 by Western Blot.
Western blot shows lysates of human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat FKBP12.6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4174) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FKBP12.6 at approximately 12.6 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat FKBP12.6 Biotinylated Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human brain (hippocampus) tissue
Sample: Human brain (hippocampus) tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FKBP12.6
FK506 binding protein, 12.6 kDa molecular weight (FKBP12.6), also called FKBP1B, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans‑ proline residues critical for proper folding of proteins. The immunosuppressant FK506 is a potent inhibitor of FKBP12.6. FKBP12.6 is localized in the sarcoplasmic reticulum associated with the Ryanodyne receptor RYR2. PKA-induced phosphorylation of RYR2 causes FKBP12.6 dissociation. In heart failure, RYR2 is hyperphosphorylated and has low levels of FKBP12.6 bound to it. Male FKBP12.6 knockout mice have cardiac hypertrophy and lethal exercise arrhythmias.
Long Name
12.6 kDa FK506 Binding Protein
Alternate Names
FKBP1B, FKBP1L, FKBP9, OTK4
Entrez Gene IDs
2281 (Human)
Gene Symbol
FKBP1B
UniProt
Additional FKBP12.6 Products
Product Documents for Human/Mouse/Rat FKBP12.6 Biotinylated Antibody
Product Specific Notices for Human/Mouse/Rat FKBP12.6 Biotinylated Antibody
For research use only
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