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Human/Mouse/Rat GATE-16 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9035

R&D Systems, part of Bio-Techne
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MAB9035
MAB9035-SP

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 853746

Product Specifications

Immunogen

E.coli-derived recombinant human GATE16
Accession # P60520

Specificity

Detects human, mouse, and rat GATE-16 in Western blot.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat GATE-16 Antibody

Detection of Human, Mouse, and Rat GATE-16 antibody by Western Blot.

Detection of Human, Mouse, and Rat GATE-16 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, mouse brain (cerebellum) tissue, and rat brain (total) tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat GATE-16 Monoclonal Antibody (Catalog # MAB9035) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for GATE-16 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat GATE-16 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, mouse brain (cerebellum) tissue, and rat brain (total) tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GATE-16

Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), also known as Apg8p2 and GABARAPL2, is a 117 amino acid (aa) polypeptide and a member of the Autophagy-related 8 (Atg8) family of proteins (1). GATE-16/Apg8p2 has 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GATE-16/Apg8p2 is best known for its role in autophagy (2,3). GATE-16/Apg8p2 covalently attaches to phosphatidylethanolamine (PE) the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome formation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).

References

  1. Shpilka, T. et al. (2011) Genome Biol. 12:226.
  2. Wang, H. et al. (1999) Nature 397:69.
  3. Leil, T.A. et al. (2004) J. Neurosci. 24:11429.
  4. Weidberg, H. et al. (2010) EMBO J. 29:1792.
  5. Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125

Long Name

Golgi-associated ATPase Enhancer of 16 kDa

Alternate Names

Apg8p2, ATG8C, FLC3A, GABARAPL2, GATE16, GEF2

Entrez Gene IDs

11345 (Human); 100042211 (Mouse); 64670 (Rat)

Gene Symbol

GABARAPL2

UniProt

Additional GATE-16 Products

Product Documents for Human/Mouse/Rat GATE-16 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat GATE-16 Antibody

For research use only

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