Human/Mouse/Rat Glutathione Peroxidase 4/GPX4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5457

R&D Systems, part of Bio-Techne
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MAB5457
MAB5457-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 565320

View all Glutathione Peroxidase 4/GPX4 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Glutathione Peroxidase 4
Gly74-Phe168
Accession # P36969

Specificity

Detects endogenous human, mouse and rat GPX4 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat Glutathione Peroxidase 4/GPX4 Antibody

Detection of Human/Mouse/Rat Glutathione Peroxidase 4 antibody by Western Blot.

Detection of Human/Mouse/Rat Glutathione Peroxidase 4 by Western Blot.

Western blot shows lysates of mouse, human, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Glutathione Peroxidase 4 Monoclonal Antibody (Catalog # MAB5457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glutathione Peroxidase 4 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse/Rat Glutathione Peroxidase 4/GPX4 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Mouse, human, and rat liver tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione Peroxidase 4/GPX4

Glutathione Peroxidase 4 (GPX4; also known as PHGPx) is a monomeric, 21 kDa member of the glutathione peroxidase family of proteins. It is widely expressed and serves to both protect cell membranes from phospholipid and cholesterol hydroperoxidases, and form an inactive enzyme structural component of the sperm mitochondrial capsule. Human GPX4 is 197 amino acids (aa) in length and contains a mitochondrial targeting sequence (aa 1‑27) and an enzymatically active selenocysteine at Sec73. During sperm maturation, the targeting sequence is cleaved, and GPX4 may form inactive covalently-linked oligomers. There are multiple splice variants. One is ubiquitously expressed, cytoplasmic, and shows an alternate start site at Met28. A second is 226 aa in length, nuclear, and shows an Arg-rich 65 aa substitution for aa 1‑28. Over amino acids 74‑168, human GPX4 shares 93% aa identity with mouse GPX4.

Alternate Names

GPX4, PHGPx, snGPx

Entrez Gene IDs

2879 (Human); 625249 (Mouse); 29328 (Rat)

Gene Symbol

GPX4

UniProt

P36969 (Human)

Additional Glutathione Peroxidase 4/GPX4 Products

Product Documents for Human/Mouse/Rat Glutathione Peroxidase 4/GPX4 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Glutathione Peroxidase 4/GPX4 Antibody

For research use only

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