Human/Mouse/Rat GRK2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB43391
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2089B
Product Specifications
Immunogen
E. coli-derived recombinant human GRK2
Pro468-Leu689
Accession # P25098
Pro468-Leu689
Accession # P25098
Specificity
Detects human GRK2 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat GRK2 Antibody
Detection of Human, Mouse, and Rat GRK2 by Western Blot.
Western blot shows lysates of THP-1 human acute monocytic leukemia cell line, Ramos human Burkitt's lymphoma cell line, LL/2 mouse Lewis lung carcinoma cell line, H4-II-E-C3 rat hepatoma cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat GRK2 Monoclonal Antibody (Catalog # MAB43391) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GRK2 at approximately 76 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human GRK2 by Simple WesternTM.
Simple Western lane view shows lysates of Ramos human Burkitt's lymphoma cell line and THP‑1 human acute monocytic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for GRK2 at approximately 80 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse/ Rat GRK2 Monoclonal Antibody (Catalog # MAB43391) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human GRK2 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HEK293T human embryonic kidney parental cell line and GRK2 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat GRK2 Monoclonal Antibody (Catalog # MAB43391) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GRK2 at approximately 76 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat GRK2 Antibody
Application
Recommended Usage
Knockout Validated
GRK2
is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in
GRK2 knockout HEK293T cell line.
Simple Western
1 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line and THP‑1 human acute monocytic leukemia cell line
Sample: Ramos human Burkitt's lymphoma cell line and THP‑1 human acute monocytic leukemia cell line
Western Blot
0.1 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line, Ramos human Burkitt's lymphoma cell line, LL/2 mouse Lewis lung carcinoma cell line, H4‑II‑E‑C3 rat hepatoma cell line, and C6 rat glioma cell line
Sample: THP‑1 human acute monocytic leukemia cell line, Ramos human Burkitt's lymphoma cell line, LL/2 mouse Lewis lung carcinoma cell line, H4‑II‑E‑C3 rat hepatoma cell line, and C6 rat glioma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GRK2
G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK2 is a member of the betaARK subfamily, and was originally termed beta‑adrenergic receptor kinase 1 ( betaARK1).
Long Name
G protein-coupled Receptor Kinase 2
Alternate Names
ADRBK1, BARK1, Beta-ARK1
Gene Symbol
GRK2
UniProt
Additional GRK2 Products
Product Documents for Human/Mouse/Rat GRK2 Antibody
Product Specific Notices for Human/Mouse/Rat GRK2 Antibody
For research use only
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