Human/Mouse/Rat Hepassocin/FGL1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1614
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Intracellular Staining by Flow Cytometry, Simple Western, Western Blot
Cited:
Mass Spectrometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Hepassocin/FGL1
Met1-Ile312
Accession # BAB70690
Met1-Ile312
Accession # BAB70690
Specificity
Detects human Hepassocin/FGL1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Hepassocin/FGL1 Antibody
Detection of Human, Mouse, and Rat Hepassocin/FGL1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/ Mouse/Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1614) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Hepassocin/FGL1 homodimer at approximately 65 kDa (as indicated). Hepassocin/FGL1 is resistant to reducing conditions (1). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Hepassocin/FGL1 in HepG2 Human Cell Line by Flow Cytometry.
HepG2 human hepatocellular carcinoma cell line was stained with Goat Anti-Human/Mouse/Rat Hepassocin/FGL1 Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF1614, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Intracellular Molecules.
Detection of Human Hepassocin/FGL1 by Simple WesternTM.
Simple Western lane view shows lysates of Hep3B human hepatocellular carcinoma cell line, human liver, loaded at 0.2 mg/mL. A specific band was detected for Hepassocin/FGL1 at approximately 41 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse/Rat Hepassocin/FGL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1614) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat Hepassocin/FGL1 Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: HepG2 Human cell line
Sample: HepG2 Human cell line
Simple Western
20 µg/mL
Sample: Hep3B human hepatocellular carcinoma cell line, human liver
Sample: Hep3B human hepatocellular carcinoma cell line, human liver
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, H4‑II‑E‑C3 rat hepatoma cell line, Hep3B human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, H4‑II‑E‑C3 rat hepatoma cell line, Hep3B human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Hepassocin/FGL1
References
- 1.Hara, H. et al. (2001) Biochim. Biophys. Acta. 1520:45.
Long Name
Fibrinogen Like 1
Alternate Names
FGL1, Fibrinogen-like-protein 1, FREP1
Gene Symbol
FGL1
UniProt
Additional Hepassocin/FGL1 Products
Product Documents for Human/Mouse/Rat Hepassocin/FGL1 Antibody
Product Specific Notices for Human/Mouse/Rat Hepassocin/FGL1 Antibody
For research use only
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