Human/Mouse/Rat HOMER1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6889
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Transgenic Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 666241
Product Specifications
Immunogen
Synthetic peptide KFGQWADSRA corresponding to aa 73-82 of human HOMER1
Accession # Q86YM7
Accession # Q86YM7
Specificity
Detects human HOMER1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat HOMER1 Antibody
Detection of Human HOMER1 by Western Blot.
Western blot shows lysates of human brain (cerebellum) tissue and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-HOMER1 Monoclonal Antibody (Catalog # MAB6889) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HOMER1 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Detection of Human and Mouse HOMER1 by Simple WesternTM.
Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for HOMER1 at approximately 51 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat HOMER1 Monoclonal Antibody (Catalog # MAB6889). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat HOMER1 Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: human brain (cerebellum) tissue
Sample: human brain (cerebellum) tissue
Western Blot
2 µg/mL
Sample: Human brain (cerebellum) tissue and IMR‑32 human neuroblastoma cell line
Sample: Human brain (cerebellum) tissue and IMR‑32 human neuroblastoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HOMER1
Long Name
Homer Protein Homolog 1
Alternate Names
HOMER1A, HOMER1B, HOMER1C, SYN47, Ves-1
Gene Symbol
HOMER1
UniProt
Additional HOMER1 Products
Product Documents for Human/Mouse/Rat HOMER1 Antibody
Product Specific Notices for Human/Mouse/Rat HOMER1 Antibody
For research use only
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