Human/Mouse/Rat HSPA2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6010
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 520608
Product Specifications
Immunogen
E. coli-derived recombinant human HSPA2
Lys529-Gly616
Accession # P54652
Lys529-Gly616
Accession # P54652
Specificity
Detects human, mouse, and rat HSPA2 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat HSPA2 Antibody
Detection of Human, Mouse, and Rat HSPA2 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HSPA2 Monoclonal Antibody (Catalog # MAB6010) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSPA2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat HSPA2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line
Sample: Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and C6 rat glioma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HSPA2
Heat shock protein A2 (HSPA2), also known as HSP70.2, is a 70 kDa testis specific protein. HSPA2 is required for spermatogenesis and functions as a chaperone for the spermatid DNA packaging proteins TP1 and TP2. HSPA2 is also expressed in cancer cells where it is normally localized to the cytoplasm but migrates to the nucleus during heat shock. Within aa 529‑616, human HSP70A2 shares 99% aa sequence identity with mouse and rat HSPA2.
Long Name
Heat Shock Protein A2
Alternate Names
HSP70-2
Gene Symbol
HSPA2
UniProt
Additional HSPA2 Products
Product Documents for Human/Mouse/Rat HSPA2 Antibody
Product Specific Notices for Human/Mouse/Rat HSPA2 Antibody
For research use only
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