Human/Mouse/Rat HSPB8 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4987
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rat
Applications
Validated:
Western Blot
Cited:
Co-Immunoprecipitation, Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 520817
Product Specifications
Immunogen
E. coli-derived recombinant human HSPB8
Ala2-Thr196
Accession # Q9UJY1
Ala2-Thr196
Accession # Q9UJY1
Specificity
Detects human, mouse, and rat HSPB8 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat HSPB8 Antibody
Detection of Human, Mouse, and Rat HSPB8 by Western Blot.
Western blot shows lysates of human, mouse, and rat heart tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSPB8 Monoclonal Antibody (Catalog # MAB4987) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSPB8 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat HSPB8 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Human, mouse, and rat heart tissue
Sample: Human, mouse, and rat heart tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HSPB8
Heat shock protein beta-8 (HSPB8, also HSP22 or CRYAC) is a 22‑25 kDa member of the small HSP (HSP20) family of proteins. It is expressed in muscle (smooth, skeletal and cardiac), and serves as a molecular chaperone. Human HSPB8 contains one alpha-crystalline domain (aa 93‑170) that mediates protein-protein interaction, and N- plus C-terminal flanking sequences that generate homodimers, homooligomers, and heterodimers with HSP27 and HSPB7. Phosphorylation at Ser57 blocks HSPB8 chaperone activity. Human HSPB8 shares 94% aa identity with both mouse and canine HSPB8. Deficiencies in HSPB8 are involved in distal motor neuropathy type 11A.
Long Name
Heat Shock Protein beta 8
Alternate Names
CMT2L, CRYAC, DHMN2, E2IG1, H11, HMN2A, HSP22
Gene Symbol
HSPB8
UniProt
Additional HSPB8 Products
Product Documents for Human/Mouse/Rat HSPB8 Antibody
Product Specific Notices for Human/Mouse/Rat HSPB8 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...