Human/Mouse/Rat IKK epsilon Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3199
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 346909
Product Specifications
Immunogen
E. coli-derived recombinant human IKK epsilon
Met1-Val716
Accession # Q14164
Met1-Val716
Accession # Q14164
Specificity
Detects human, mouse, and rat IKK epsilon.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat IKK epsilon Antibody
Detection of Human IKK epsilon by Western Blot.
Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Ramos human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat IKKe Monoclonal Antibody (Catalog # MAB3199) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IKKe at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
IKK epsilon in MCF-7 Human Cell Line.
IKKe was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat IKKe Monoclonal Antibody (Catalog # MAB3199) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
IKK epsilon in Human PBMCs.
IKKe was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS and monensin using Mouse Anti-Human/Mouse/Rat IKKe Monoclonal Antibody (Catalog # MAB3199) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Mouse/Rat IKK epsilon Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells stimulated with LPS and monensin, and immersion fixed MCF‑7 human breast cancer cell line
Sample: Immersion fixed human peripheral blood mononuclear cells stimulated with LPS and monensin, and immersion fixed MCF‑7 human breast cancer cell line
Western Blot
1 µg/mL
Sample: MDA-MB-468 human breast cancer cell line, Ramos human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line
Sample: MDA-MB-468 human breast cancer cell line, Ramos human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IKK epsilon
I kappaB kinase epsilon (IKK epsilon) is also known as IKBKE and IKKi. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappaB, resulting in the release and nuclear translocation of active NF-kappa B. IKK epsilon is an IKK-related kinase with 30% structural homology to IKK alpha and beta, and is present in a subset of IKK complexes. IKK epsilon activation has been implicated in the initiation of anti-viral gene expression and in the pathogenesis of breast cancer.
Long Name
IkB Kinase epsilon
Alternate Names
IKBKE, IKK-I
Gene Symbol
IKBKE
UniProt
Additional IKK epsilon Products
Product Documents for Human/Mouse/Rat IKK epsilon Antibody
Product Specific Notices for Human/Mouse/Rat IKK epsilon Antibody
For research use only
Loading...
Loading...
Loading...
Loading...