Detects human, mouse, and rat Jagged 1. In direct ELISAs, no cross‑reactivity with recombinant human Jagged 2 is observed. Stains human Jagged 1 transfectants but not the parental cell line or human Jagged 2 transfectants in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Antibody

Detection of Jagged 1 in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line was stained with Mouse Anti-Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1277F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Jagged 1 in Rat Cortical Stem Cells by Flow Cytometry.

Rat cortical stem cells were stained with Mouse Anti-Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1277F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: bEnd.3 Mouse Endothelioma Cell Line and Rat Cortical Stem Cells

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region. The ECD contains a DSL domain (aa 185‑229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 shares 97% and 96% aa identity with mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9, 10).

References

Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.
Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.
Artavamis-Tsokanas, S. et al. (1999) Science 284:770.
Lai, E.C. (2004) Development 131:965.
Milner, L.A. et al. (1994) Blood 83:2057.
Nyfeler, Y. et al. (2005) EMBO J. 24:3504
Linheng, L. et al. (1998) Immunity 8:43.
Iso, T. et al. (2003) Arterioscler. Thromb. Vasc. Biol. 23:543.
Oda, T. et al. (1997) Nat. Genet. 16:235.
Oda, T. et al. (1997) Genomics 43:376.

Alternate Names

CD339, JAG1

Entrez Gene IDs

182 (Human); 16449 (Mouse); 29146 (Rat)

Gene Symbol

JAG1

UniProt

P78504 (Human)

Additional Jagged 1 Products

Product Documents for Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human/Mouse/Rat Jagged 1 Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1277F

Key Product Details

Species Reactivity

Validated:

Cited:

Applications