Human/Mouse/Rat Jak1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4260
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
CyTOF-ready, Immunocytochemistry, Immunohistochemistry, Intracellular Staining by Flow Cytometry, Simple Western, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 413104
Product Specifications
Immunogen
E. coli-derived recombinant human Jak1
Pro32-Phe286
Accession # P23458
Pro32-Phe286
Accession # P23458
Specificity
Detects human, mouse, and rat Jak1.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat Jak1 Antibody
Detection of Jak1 in Jurkat Human Cell Line by Flow Cytometry.
Jurkat human acute T cell leukemia cell line was stained with Rat Anti-Human/Mouse/Rat Jak1 Monoclonal Antibody (Catalog # MAB4260, filled histogram) or isotype control antibody (MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Detection of Human and Mouse Jak1 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, A20 mouse B cell lymphoma cell line, and L1.2 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat Jak1 Monoclonal Antibody (Catalog # MAB4260) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Jak1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Jak1 in Human Epidermis.
Jak1 was detected in immersion fixed paraffin-embedded sections of human epidermis using 25 µg/mL Rat Anti-Human/Mouse/Rat Jak1 Monoclonal Antibody (Catalog # MAB4260) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat Jak1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human epidermis
Sample: Immersion fixed paraffin-embedded sections of human epidermis
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin
Simple Western
10 µg/mL
Sample: Lysates of Daudi human Burkitt's lymphoma cell line.
Sample: Lysates of Daudi human Burkitt's lymphoma cell line.
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, A20 mouse B cell lymphoma cell line, and L1.2 mouse pro-B cell line
Sample: Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, A20 mouse B cell lymphoma cell line, and L1.2 mouse pro-B cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 5 using MAB4260 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Jak1
Janus Kinase 1 (Jak1) belongs to a family of protein tyrosine kinases that couple to cytokine receptors and are activated by ligand binding to these receptors. Activation of Jak1 occurs via phosphorylation at two adjacent tyrosine residues, Y1022 and Y1023, within the kinase domain. Jaks activate members of the STAT family of transcription factors by phosphorylating critical tyrosine regulatory sites. Jak1 is required for the activation of STAT1 and STAT2 in response to interferon alpha.
Long Name
Janus Kinase 1
Alternate Names
EC 2.7.10, JAK-1, JAK1Atyrosine-protein kinase JAK1, JAK1B, Janus kinase 1EC 2.7.10.2, JTK3
Entrez Gene IDs
3716 (Human)
Gene Symbol
JAK1
UniProt
Additional Jak1 Products
Product Documents for Human/Mouse/Rat Jak1 Antibody
Product Specific Notices for Human/Mouse/Rat Jak1 Antibody
This product is sold under license from Millipore Corporation under the following US or foreign patents: 5,821,069; 5,658,791; EP0560890. This product shall not be used to commercially screen drug molecules being developed as JAK1 or JAK2 inhibitors. Any such activity will be outside the scope of the research use only label license.
For research use only
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