Human/Mouse/Rat JNK Pan Specific Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1387
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 252355
Product Specifications
Immunogen
E. coli-derived recombinant human JNK
Ser2-Ile384
Accession # P45983
Ser2-Ile384
Accession # P45983
Specificity
Detects human, mouse, and rat p46 and p54 JNK in Western blots. Detects recombinant JNK1, JNK2, and JNK3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat JNK Pan Specific Antibody
Detection of Human/Mouse JNK by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK Pan Specific Monoclonal Antibody (Catalog # MAB1387) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands for JNK were detected at approximately 46 kDa (p46 JNK) and 54 kDa (p54 JNK) (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human JNK by Western Blot.
Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK Pan Specific Monoclonal Antibody (Catalog # MAB1387) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human JNK by Simple WesternTM.
Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for JNK at approximately 47 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat JNK Pan Specific Monoclonal Antibody (Catalog # MAB1387) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat JNK Pan Specific Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: HEK293T human embryonic kidney cell line
Sample: HEK293T human embryonic kidney cell line
Western Blot
0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and C2C12 mouse myoblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and C2C12 mouse myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: JNK
Long Name
C-Jun N-terminal Kinase
Alternate Names
c-Jun N-terminal kinase 1, EC 2.7.11, EC 2.7.11.24, JNK1 alpha protein kinase, JNK1 beta protein kinase, JNK1JNK1A2, JNK-46, JUN N-terminal kinase, MAP kinase 8, MAPK 8, mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-activated protein kinase 8 isoform JNK1 beta2, PRKM8JNK, protein kinase JNK1, SAPK1JNK21B1/2, Stress-activated protein kinase 1, Stress-activated protein kinase JNK1
UniProt
Additional JNK Products
Product Documents for Human/Mouse/Rat JNK Pan Specific Antibody
Product Specific Notices for Human/Mouse/Rat JNK Pan Specific Antibody
For research use only
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