Human/Mouse/Rat JNK1/JNK2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2076
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 252323
Product Specifications
Immunogen
E. coli-derived recombinant human JNK1 isoform 2
Specificity
Detects human, mouse, and rat p46 and p54 JNK1/JNK2. Does not detect recombinant JNK3.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat JNK1/JNK2 Antibody
Detection of Human/Mouse/Rat JNK1/JNK2 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human JNK1, JNK2, and JNK3 (1 ng/lane) were included. Specific bands for JNK1 and JNK2 were detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
JNK1/JNK2 in HeLa Human Cervical Epithelial Carcinoma Cell Line.
JNK1/JNK2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
JNK1/JNK2 in C2C12 Mouse Cell Line.
JNK1/JNK2 was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human/Mouse/Rat JNK1/JNK2 Monoclonal Antibody (Catalog # MAB2076) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Mouse/Rat JNK1/JNK2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line
Simple Western
10 µg/mL
Sample: HEK293T human embryonic kidney cell line
Sample: HEK293T human embryonic kidney cell line
Western Blot
0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: JNK1/JNK2
Long Name
C-Jun N-terminal Kinase
Alternate Names
c-Jun N-terminal kinase 1, JNK, JNK1, JNK1A2, JNK21B1/2, JNK-46, JUN N-terminal kinase, MAP kinase 8, mitogen-activated protein kinase 8, PRKM8, SAPK1, SAPK1C, Stress-activated protein kinase 1, stress-activated protein kinase 1c
Additional JNK1/JNK2 Products
Product Documents for Human/Mouse/Rat JNK1/JNK2 Antibody
Product Specific Notices for Human/Mouse/Rat JNK1/JNK2 Antibody
For research use only
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