Human/Mouse/Rat MEK2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2855
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 300317
Product Specifications
Immunogen
E. coli-derived recombinant human MEK2
Met1-Val400
Accession # P36507
Met1-Val400
Accession # P36507
Specificity
Detects human, mouse, and rat MEK2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) MEK1 or rhMEK5 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat MEK2 Antibody
Detection of Human/Mouse/Rat MEK2 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat MEK2 Monoclonal Antibody (Catalog # MAB2855) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MEK1 and Recombinant Human Active MEK2 (Catalog # 2855-KS) (5 ng/lane) were included. A specific band for MEK2 was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
MEK2 in HeLa Human Cell Line.
MEK2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat MEK2 Monoclonal Antibody (Catalog # MAB2855) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat MEK2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line
Western Blot
0.5 µg/mL
Sample: MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell
Sample: MCF-7 human breast cancer cell line, U937 human histiocytic lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB2855 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MEK2
Long Name
Mitogen-activated Protein Kinase Kinase 2
Alternate Names
MAP2K2, MKK2
Gene Symbol
MAP2K2
UniProt
Additional MEK2 Products
Product Documents for Human/Mouse/Rat MEK2 Antibody
Product Specific Notices for Human/Mouse/Rat MEK2 Antibody
For research use only
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