Human/Mouse/Rat MEKK2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7128
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 722728
Product Summary for Human/Mouse/Rat MEKK2 Antibody
Immunogen
E. coli-derived recombinant human MEKK2
Asn42-Tyr264
Accession # Q9Y2U5
Asn42-Tyr264
Accession # Q9Y2U5
Specificity
Detects recombinant human MEKK2 in direct ELISA and human, mouse, rat MEKK2 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human MEKK1, 3, or 4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat MEKK2 Antibody
Detection of Human, Mouse, and Rat MEKK2 by Western Blot.
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, Saos-2 human osteosarcoma cell line, BJAB human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat MEKK2 Monoclonal Antibody (Catalog # MAB7128) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MEKK2 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
MEKK2 in 293T Human Cell Line.
MEKK2 was detected in immersion fixed 293T human embryonic kidney cell line using Mouse Anti-Human/Mouse/Rat MEKK2 Monoclonal Antibody (Catalog # MAB7128) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. This application has not been tested in mouse or rat samples.Applications for Human/Mouse/Rat MEKK2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed 293T human embryonic kidney cell line
Sample: Immersion fixed 293T human embryonic kidney cell line
Western Blot
2 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line, Saos‑2 human osteosarcoma cell line, BJAB human Burkitt's lymphoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: Ramos human Burkitt's lymphoma cell line, Saos‑2 human osteosarcoma cell line, BJAB human Burkitt's lymphoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Rat‑2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB7128 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MEKK2
Long Name
Mitogen-activated Protein Kinase Kinase Kinase 2
Alternate Names
MAP3K2, MAPKKK2
Gene Symbol
MAP3K2
UniProt
Additional MEKK2 Products
Product Documents for Human/Mouse/Rat MEKK2 Antibody
Product Specific Notices for Human/Mouse/Rat MEKK2 Antibody
For research use only
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