Human/Mouse/Rat MKP-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3576
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 363246
Product Specifications
Immunogen
E. coli-derived recombinant human MKP-3
Ile2-Thr381
Accession # Q16828
Ile2-Thr381
Accession # Q16828
Specificity
Detects endogenous human, mouse, and rat MKP-3 in Western blots. In Western blots, this antibody does not cross-react with recombinant human MKP-1 or MKP-2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat MKP-3 Antibody
Detection of Human and Mouse MKP-3 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat MKP-3 Monoclonal Antibody (Catalog # MAB3576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MKP-3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat MKP-3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, CTLL-2 mouse cytotoxic T cell line, and C2C12 mouse myoblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, CTLL-2 mouse cytotoxic T cell line, and C2C12 mouse myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MKP-3
MAP Kinase Phosphatase 3 (MKP-3), also called PYST1 and Dual Specificity Phosphatase 6 (DUSP6), dephosphorylates serine, threonine, and tyrosine residues in proteins. On Western blots, MKP-3 has an apparent molecular weight of 42 kilodaltons. It has a high degree of substrate selectivity for dephosphorylating ERK kinases over stress-related kinases such as p38 and JNK. MKP-3 is ubiquitinylated prior to degradation in the proteosome. The rate of MKP-3 degradation is enhanced by ERK-mediated phosphorylation.
Long Name
MAP Kinase Phosphatase 3
Alternate Names
DUSP6, MKP3, PYST1
Entrez Gene IDs
1848 (Human)
Gene Symbol
DUSP6
UniProt
Additional MKP-3 Products
Product Documents for Human/Mouse/Rat MKP-3 Antibody
Product Specific Notices for Human/Mouse/Rat MKP-3 Antibody
For research use only
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