Human/Mouse/Rat N-WASP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3854
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human N-WASP
Leu21-Asn270
Accession # O00401
Leu21-Asn270
Accession # O00401
Specificity
Detects human, mouse, and rat N-WASP in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat N-WASP Antibody
Detection of Human, Mouse, and Rat N-WASP by Western Blot.
Western blot shows lysates of DU145 human prostate carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat N-WASP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3854) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for N-WASP at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
N‑WASP in Rat Brain Tissue.
N-WASP was detected in perfusion fixed frozen sections of rat brain (cortex) tissue using Goat Anti-Human/Mouse/Rat N-WASP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3854) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Human/Mouse/Rat N-WASP Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line treated with recombinant human EGF (Catalog # 236-EG-01M)
Sample: Immersion fixed A431 human epithelial carcinoma cell line treated with recombinant human EGF (Catalog # 236-EG-01M)
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (cortex)
Sample: Perfusion fixed frozen sections of rat brain (cortex)
Western Blot
1 µg/mL
Sample: DU145 human prostate carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line
Sample: DU145 human prostate carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and PC-12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: N-WASP
Long Name
Neural Wiskott-Aldrich Syndrome Protein
Alternate Names
NWASP, TRS4, WASL
Gene Symbol
WASL
UniProt
Additional N-WASP Products
Product Documents for Human/Mouse/Rat N-WASP Antibody
Product Specific Notices for Human/Mouse/Rat N-WASP Antibody
For research use only
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