Human/Mouse/Rat Ninjurin-2 Antibody

Detection of Ninjurin-2 in Human Tonsil

Ninjurin-2 was detected in immersion fixed paraffin-embedded sections of Human Tonsil using Sheep Anti-Human/Mouse/Rat Ninjurin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5056) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface on lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human/Mouse/Rat Ninjurin-2 by Western Blot.

Western blot shows lysates of human thymus, mouse thymus, rat thymus, NTera-2 human testicular embryonic carcinoma cells and HT-29 human colon adenocarcinoma cells. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Ninjurin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5056) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ninjurin-2 at approximately ~26kDa kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.