Human/Mouse/Rat p27/Kip1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2256
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rat
Applications
Validated:
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 225501
Product Specifications
Immunogen
E. coli-derived recombinant human p27
Met1-Thr198
Accession # P46527
Met1-Thr198
Accession # P46527
Specificity
Detects human, mouse, and rat p27 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat p27/Kip1 Antibody
Detection of Human/Mouse/Rat p27/Kip1 by Western Blot.
Western blot shows lysates of NRK rat normal kidney cell line, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat p27/Kip1 Monoclonal Antibody (Catalog # MAB2256) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p27/Kip1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat p27/Kip1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin
Sample: Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin
Western Blot
0.2 µg/mL
Sample: NRK rat normal kidney cell, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line
Sample: NRK rat normal kidney cell, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p27/Kip1
The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle.
Long Name
p27 Cyclin Dependent Kinase 4 Inhibitor 1B
Alternate Names
CDKN1B, Kip1
Gene Symbol
CDKN1B
UniProt
Additional p27/Kip1 Products
Product Documents for Human/Mouse/Rat p27/Kip1 Antibody
Product Specific Notices for Human/Mouse/Rat p27/Kip1 Antibody
For research use only
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